David Spencer (Holbrook Blinn) is a basically good man, but like all men he has a few character flaws. Alas, these flaws deepen into weaknesses, leading to disaster for Spencer and his lady love Janice Lane (Eleanor Woodruff).

Jaffery Chayne is the spectacular one of four chums, the others being Hilary Freeth, a literary man, Adrian Boldero, a short story writer, and Tom Castleton, a playwright. The story opens with Tom Castleton going on a voyage for his health and leaving with his friend, Adrian, the manuscript of the first novel he ever attempted. Shortly after Castleton's trip, he dies at sea and when word is received by Adrian of his friend's death, the temptation to secure the girl he loves by publishing his friend's novel and taking the money and credit from it is so strong that he succumbs and becomes the "literary lion of the hour."

Big Jim Garrity, a mine superintendent, is falsely accused of murder by a dope doctor and is forced into exile in Europe. After several years, he has made a fortune and returns to the U.S. under an assumed name. He makes his way into society, where he falls in love with the girl whose brother he is accused of murdering.

Lieutenant Commander Colton, U.S.N., is in love with Caroline Austen, daughter of a prominent political power in Washington. Colton has a rival in James Archer, a journalist of prominence, unscrupulous and secretly in league with the Ruanian Ambassador, who is endeavoring to obtain for his country inside information as to the United States naval resources.

While traveling incognito through his kingdom, Prince Ludwig of Saxe-Tholberg becomes infatuated with Katrina, the daughter of innkeeper Hermann Ardelheim, but their idyll is interrupted by the arrival of a courier bearing the news that Austrania has threatened war. Katrina is heartbroken to discover the identity of her sweetheart whom she can never hope to marry. After Ludwig's departure, Katrina overhears the plotting of two spies and with the help of her brother Roalf, she confronts them. In the ensuing struggle, Katrina kills one of them, who turns out to be the Austranian ambassador. Although Katrina admits her crime, Marshal von Trump plans to execute Roalf in order to pacify the Austranians. Katrina appeals to Ludwig, who pardons Roalf over the advice of his counselors causing Austrania to declare war. A crucial battle is fought near the Ardelheim inn, during which Katrina becomes a heroine by signaling the advance of Ludwig's troops.

An ambitious bank teller (Edward Jose) steals a large deposit and starts life over under an assumed name. While he is becoming a lawyer and making his way up the ladder of success with the help of a political boss, the wife he left behind (Eleanor Woodruff) remains destitute and is forced to give up her child to an orphanage. The girl is adopted and grows up (played as an adult by Virginia Pearson) to become the secretary to an honest young lawyer. But the girl has the same quirk that her father had, and it causes her to steal a bracelet at a department store. She is arrested and finds herself before her father, who is now a judge.

The Perils of Pauline is a motion picture serial shown in weekly installments featuring the actress Pearl White playing the title character. Pauline has often been cited as a famous example of a damsel-in-distress, although viewers will find her character more resourceful and less helpless than the classic 'damsel' stereotype. Nine episodes (from a condensed 1916 re-release) survive to this day.