The film follows Henson’s career from his first work with his Muppets in the early 1950’s during television’s Golden Age up to his sudden and shocking death in 1990, as he transcended puppetry to become one of the most creative, prolific and influential artists of his time. Key events include his early television work with the Muppets while still a student at the University of Maryland, his commercial work and network appearances in the late 1950s and early 1960’s, his breakthrough with Sesame Street, The Muppet Show, Fraggle Rock, and his groundbreaking fantasy films, Dark Crystal and Labyrinth. Among those interviewed for the Jim Henson episode are his children, Lisa, Cheryl, Brian and Heather Henson, actors Neil Patrick Harris and Candice Bergen, Willard Scott, director John Landis, former Disney CEO Michael Eisner, and Sesame Street cast members Bob McGrath and Carroll Spinney.

Seymour Krelborn is a nerdy orphan working at Mushnik's, a flower shop in urban Skid Row. He harbors a crush on fellow co-worker Audrey Fulquard, and is berated by Mr. Mushnik daily. One day Seymour finds a very mysterious unidentified plant which he calls Audrey II. The plant seems to have a craving for blood and soon begins to sing for his supper.

Two bumbling government employees think they are U.S. spies, only to discover that they are actually decoys for nuclear war.

When the Muppets graduate from Danhurst College, they take their song-filled senior revue to New York City, only to learn that it isn't easy to find a producer who's willing to back a show starring a frog and a pig. Of course, Kermit the Frog and Miss Piggy won't take no for an answer, launching a search for someone to take them to Broadway.

A Hollywood agent persuades Kermit the Frog to pursue a career in Hollywood. On his way there he meets his future muppet crew while being chased by the desperate owner of a frog-leg restaurant!

A retelling of the classic fairy tale of The Frog Prince featuring Kermit the Frog, Kermit's nephew Robin, Sweetums, and a princess cursed to say everything wackbards...I mean backwards.