Flikken Rotterdam takes place in Rotterdam, and is after Flikken Maastricht the second Dutch spin-off of the Flemish show Flikken.

After losing sight of each other for 25 years, the paths of three childhood friends cross paths. It concerns successful conductor Victor Slingeland, successful writer Sander Vastenhout and general practitioner Bert Duprez. Slingeland enjoys a reputation as a womanizer. This intrigues Vastenhout who, as is often the case with authors, is without inspiration. Vastenhout sees the unraveling of Slingeland's complex personality as possible new material for a novel. With the help of Dr. Duprez, Vastenhout tries to find out about Slingeland's secret by imitating his behavior and thus challenging him.