Vampire fledgling Michelle Morgan has escaped the grasp of her master Radu Vladislas. Found by a woman named Ana, she is taken to a hospital where a doctor claims to be able to cure her vampirism. Radu, recovering from the near-death delivered by Michelle and her friends, travels to Bucharest to follow his fledgling. He visits the vampire Ash's stronghold. Ash and his protege Serena plot to destroy Radu and employ the help of the humans, Ana and the Doctor.

Still in the thrall of the evil vampire Radu, Michelle yearns to be taught the skills of the vampire. Meanwhile, her sister Becky tries to free her from his evil clutches, and this time, she's brought some help.

Continuing after the first "Subspecies", a woman who has just become a vampire tries to escape the evil vampire, Radu, who seeks her as his love interest. But she has taken the vampire family's bloodstone, and now Radu must find her to get it back. While her sister comes to Romania to save her soul. It might be too late....