Peppino, a retired hitman for the Camorra, has now fully passed on his job and know-how to his single son, Nino. But when Nino is brutally assassinated, the old man is back in business to take revenge. Aside his everlasting love Rita and his longtime henchman Totò, Peppino will go to any lengths, even if it means bringing the Camorra down.

Taking place after the events of Assassin's Creed: Revelations in the year 1524 in Tuscany, follow the retired Assassin Ezio Auditore as he teams up with Shao Jun during his final battles.