In the lawless West, The Cowboys, a notorious brotherhood of killers and thieves, reigned over the land with brutal fists and fast guns. Fate had finally caught up with them and now the merciless gang has but a single surviving member. When a deputized gunslinger takes up the call to hunt down the last Cowboy, the chase is on and the bullets fly, and only one of these hardened men can survive.

Harley, a runaway thoroughbred, is found by a rancher and his daughter. Unable to find the owner, they nurture him back to health, and the girl includes Harley as her partner in her horse club competitions.